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Plasma microRNAs serve as potential biomarkers for abdominal aortic aneurysm
Wenwen Zhang1, Tao Shang1, Chen Huang1
1Department of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, PR China.
Objectives:
Abdominal aortic aneurysm (AAA) is often asymptomatic until rupture occurs. Although ultrasound screening has significantly improved the early diagnosis of AAA, the timely biomarker-based diagnosis of AAA remains a major clinical challenge. In this study, we aimed to assess plasma microRNAs (miRNAs) as promising novel biomarkers in patients with AAA.
Methods:
Pooled plasma samples from 10 AAA and 10 healthy controls were profiled by microarray. The differentially expressed miRNAs were evaluated in a separate cohort of 120 subjects, including 60 AAA patients and 60 normal controls.
Results:
The initial profiling study identified 151 miRNAs that showed more than two-fold change. Among them, three miRNAs, miR-191-3p, miR-455-3p and miR-1281 exhibited the largest increase in the patient group (fold change>5). A subsequent validation study confirmed the elevation of these three miRNAs. Receiver operator characteristic curve analysis using the expression ratio of miR-191-3p, miR-455-3p and miR-1281 showed an area under the curve of 0.9700, 0.9825 and 0.9206, respectively.
Conclusion:
Our results suggest that plasma miR-191-3p, miR-455-3p and miR-1281 may be used as potential diagnosis biomarkers for AAA.
Insights
Three plasma microRNAs (miRNAs) show elevated levels in patients with abdominal aortic aneurysm (AAA). These specific miRNAs, miR-191-3p, miR-455-3p, and miR-1281, could serve as novel diagnostic biomarkers for AAA.
Area of Science:
- Cardiovascular Research
- Biomarker Discovery
- Molecular Diagnostics
Background:
- Abdominal aortic aneurysm (AAA) diagnosis is challenging due to its asymptomatic nature until rupture.
- Current screening methods like ultrasound aid early detection, but timely biomarker-based diagnosis remains a clinical hurdle.
- Plasma microRNAs (miRNAs) are being investigated as potential novel biomarkers for various diseases.
Purpose of the Study:
- To investigate the potential of plasma microRNAs (miRNAs) as diagnostic biomarkers for abdominal aortic aneurysm (AAA).
- To identify specific miRNAs that are differentially expressed in patients with AAA compared to healthy controls.
Main Methods:
- Microarray profiling of pooled plasma samples from 10 AAA patients and 10 healthy controls.
- Validation of differentially expressed miRNAs in a larger cohort of 60 AAA patients and 60 normal controls.
- Receiver operator characteristic (ROC) curve analysis to assess diagnostic accuracy.
Main Results:
- Initial profiling identified 151 differentially expressed miRNAs, with miR-191-3p, miR-455-3p, and miR-1281 showing the most significant increase (fold change > 5).
- Validation confirmed the elevated expression of these three miRNAs in AAA patients.
- ROC analysis demonstrated high diagnostic potential, with areas under the curve of 0.9700 for miR-191-3p, 0.9825 for miR-455-3p, and 0.9206 for miR-1281.
Conclusions:
- Plasma miR-191-3p, miR-455-3p, and miR-1281 show promise as potential diagnostic biomarkers for abdominal aortic aneurysm (AAA).
- These miRNAs could contribute to improved early diagnosis and management of AAA.
- Further research is warranted to validate these findings in larger, diverse populations.
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