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Related Experiment Videos

Purinergic modulation in the flounder gut.

R Lennard1, H Huddart

  • 1Institute of Environmental and Biological Sciences, University of Lancaster, Bailrigg, England.

General Pharmacology
|January 1, 1989
PubMed
Summary

Flounder ileum and rectum responses to ATP and adenosine were studied. ATP acts via P2 receptors, while adenosine effects involve mixed P1 purinoreceptors in these fish tissues.

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Area of Science:

  • Pharmacology
  • Comparative Physiology
  • Gastrointestinal Physiology

Background:

  • The gastrointestinal tract's mechanical activity is crucial for nutrient absorption.
  • Purinergic signaling, involving adenosine triphosphate (ATP) and adenosine, plays a significant role in regulating smooth muscle function.
  • Understanding these mechanisms in marine species like the flounder provides insights into vertebrate evolution.

Purpose of the Study:

  • To investigate the effects of ATP and adenosine on the mechanical activity of the flounder (Platichthys flesus) ileum and rectum.
  • To identify the specific purinoreceptors involved in mediating these responses.

Main Methods:

  • Isometric recordings of spontaneous mechanical responses from isolated ileum and rectum segments of the flounder.
  • Dose-response experiments using ATP and adenosine.
  • Pharmacological characterization using the P2 receptor antagonist alpha,beta-meATP and the P1 receptor antagonist theophylline.

Main Results:

  • ATP induced dose-dependent positive inotropic responses in both ileum and rectum, without affecting chronotropic activity.
  • The P2 receptor antagonist alpha,beta-meATP caused contractures and blocked ATP-induced responses, indicating P2 receptor mediation.
  • Adenosine abolished ileal rhythmic activity and lowered basal tonus but caused positive inotropic responses in the rectum.
  • Theophylline blocked adenosine's inotropic effects in the rectum and its inhibitory actions in the ileum.

Conclusions:

  • The positive mechanical effects of ATP on the flounder ileum and rectum are mediated by P2 purinoreceptors.
  • Adenosine elicits differential responses in the flounder ileum (inhibition) and rectum (excitation), suggesting the involvement of mixed populations of P1 purinoreceptors.

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