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Gas Chromatography: Types of Detectors-II

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Related Experiment Video

Updated: Apr 14, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

A new simple chromo-fluorogenic probe for NO2 detection in air.

L Alberto Juárez^1,2, Ana M Costero^3,4, Félix Sancenón^1,5,6

¹Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universidad Politécnica de Valencia, Universidad de Valencia (Spain).

Chemistry (Weinheim an Der Bergstrasse, Germany)

|April 29, 2015

Summary

A novel biphenyl-based probe detects nitrogen dioxide (NO2) in gas phase, changing color and quenching fluorescence. This sensitive method achieves a 0.1 ppm detection limit for atmospheric NO2 monitoring.

Keywords:

NO2 chromogenic detection gas phase reactions sensors

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Area of Science:

Chemical sensing
Analytical chemistry
Materials science

Background:

Nitrogen dioxide (NO2) is a harmful air pollutant.
Accurate and sensitive detection of NO2 is crucial for environmental monitoring.
Existing detection methods may lack sensitivity or require complex instrumentation.

Purpose of the Study:

To develop a new chromo-fluorogenic probe for NO2 detection.
To evaluate the probe's performance in gas-phase NO2 sensing.
To assess the probe's applicability in real-world atmospheric conditions.

Main Methods:

Synthesis of a biphenyl derivative probe with silylbenzyl ether and N,N-dimethylamino groups.
Observation of colorimetric and fluorometric changes upon reaction with NO2.
Determination of the limit of detection (LOD) using visual assessment.
Testing the probe in simulated and actual atmospheric environments.

Main Results:

The probe exhibited a distinct color change from colorless to yellow upon exposure to NO2.
Fluorescence emission of the probe was quenched in the presence of NO2.
A low limit of detection of approximately 0.1 ppm was achieved for naked-eye detection.
Successful application of the probe for NO2 detection under realistic atmospheric conditions.

Conclusions:

The developed biphenyl-based probe is effective for sensitive gas-phase NO2 detection.
The probe offers a simple and visual method for monitoring NO2 levels.
This technology holds promise for environmental air quality assessment.