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Updated: Apr 14, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
Published on: June 19, 2015
Resistance-Gene Cassettes Associated With Salmonella enterica Genotypes
Bita Bakhshi1, Mohsen Ghafari2, Mohammad R Pourshafie3
1Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran b.bakhshi@modares.ac.ir.
Background:
The epidemiology of salmonellosis is complex because of the diversity and different serotypes of Salmonella enterica (S. enterica) that occur in different reservoirs and geographic incidences.
Objectives:
To determine the genotype distribution and resistance-gene content of 2 classes of integron among S. enterica isolates.
Methods:
Thirty-six S. enterica species were isolated and tested for their serological distribution and the resistance-gene contents of 2 classes of integron, as well as for their genetic diversity, using the pulsed-field gel electrophoresis (PFGE) genotyping method.
Results:
Serogroups E (36.1%) and D (30.5%) were dominant among the isolates. All of the isolates in serogroup D belonged to the serovar enteritidis. The aadA1 gene was found within all resistance-gene cassettes. We observed 4 common and 26 single pulsotypes among the isolates, which indicated a high degree of genetic diversity among the isolates. Using the PulseNet International standard protocol, it was found that these isolates were different from those reported previously in Iran.
Conclusions:
The presence of a few common and new pulsotypes among the isolates suggests the emergence and spread of new clones of S. enterica in Iran.
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