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Hamster oocyte penetration tests with oocytes frozen in propanediol: comparison with non-frozen oocytes
H H Sachs1, M J Pink, R B Gwatkin
1Reproductive and Developmental Biology, Research Institute and Gynecology, Cleveland Clinic Foundation, Ohio.
Gamete Research
|September 1, 1989
Abstract:
Hamster oocytes were frozen using a 1,2-propanediol-sucrose procedure, which resulted in over 90% survival. After thawing and zona removal the oocytes were compared with non-frozen oocytes in a zona-free hamster egg test employing spermatozoa from human semen donors and suspected infertility patients. Similar data were obtained, indicating that propanediol-sucrose frozen hamster eggs may be used in place of fresh eggs for convenience and to avoid scheduling problems.