Related Experiment Videos
Molecular cloning and chromosomal assignment of a human perforin (PFP) gene
Y Shinkai1, M C Yoshida, K Maeda
1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Immunogenetics
|January 1, 1989
Abstract:
Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that the human perforin (PFP) gene is located on chromosome 17q11-21.