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Published on: October 9, 2014
Effect of soft-tissue attachment on tibial fracture healing in rats
Olav Reikeras1, Mona I Winge, Magne Røkkum
1Department of Orthopaedic Surgery, Rikshospitalet Oslo University Hospital, Oslo, Norway.
Purpose:
To compare tibial fracture healing in rats with or without soft-tissue attachment.
Methods:
The left tibias of 30 Wistar rats were osteotomised and equally randomised into 3 groups. In the avascular segmental fracture group, an 8-mm bone segment were first removed and then immediately put back. In the vascular segmental fracture group, the 8-mm bone segment was not displaced, with periosteal and muscular attachments. In the simple fracture group, a simple fracture in the middle tibia was made. All tibias were then stabilised with an intramedullary nail through the patellar tendon, and the wound was closed with sutures. After 8 weeks, all left tibias and 9 of the intact right tibias were harvested. Bone mineral content and density of the calluses were assessed using dual energy X-ray absorptiometry scanning. The maximum torsional strength, rigidity, and energy to failure of the tibias were measured.
Results:
All tibias healed (callus formation), without mal-union or pin migration. The 3 groups did not differ significantly in terms of torsional strength, rigidity, energy to failure, bone mineral content, and bone mineral density. The mean torsional strength of the 9 intact tibias was significantly higher than the healed tibias (14.9 vs. 10.6 Nmm, p=0.021).
Conclusion:
Soft-tissue detachment from bone segments did not impair bone healing in rats.
