Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphonuclear leukocyte stimulation measured by phage inactivation.

U Ferrini1, A M Mileo, A Nista

  • 1Laboratory of Biophysics, Regina Elena Institute for Cancer Research, Rome, Italy.

International Archives of Allergy and Applied Immunology
|January 1, 1989
PubMed
Summary

A new assay quantifies polymorphonuclear neutrophil (PMN) stimulation by measuring lambda phage inactivation. This sensitive method offers advantages for studying PMN activation processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interoception, blood pressure, and pain: unraveling their interaction under stressful and resting conditions.

Stress (Amsterdam, Netherlands)·2026
Same author

Radar evidence of subglacial liquid water on Mars.

Science (New York, N.Y.)·2018
Same author

A telemonitoring platform for the investigation of blood pressure profiles in pacemaker patients.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2017
Same author

Pacemaker and ICD oversensing induced by movements near the MRI scanner bore.

Medical physics·2016
Same author

Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53.

Cell death & disease·2015
Same author

An optically coupled system for quantitative monitoring of MRI gradient currents induced into endocardial leads.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013

Area of Science:

  • Immunology
  • Microbiology

Background:

  • Polymorphonuclear neutrophils (PMNs) play a crucial role in the innate immune response.
  • Accurate quantification of PMN stimulation is essential for understanding immune function and disease pathogenesis.

Purpose of the Study:

  • To describe a novel quantitative assay for measuring PMN stimulation.
  • To highlight the advantages of this assay compared to existing methods.

Main Methods:

  • The assay utilizes the inactivation of lambda vector phages upon exposure to stimulated PMNs.
  • Quantification is achieved by determining the number of residual plaque-forming units on a bacterial host.

Main Results:

  • The developed method provides a reproducible and sensitive assay for PMN stimulation levels.

Related Experiment Videos

  • The assay demonstrates advantages over methods using bacteria or eukaryotic cells.

    • Conclusions:

    • This phage inactivation assay offers a sensitive and reproducible method for quantifying PMN stimulation.
    • The assay is valuable for investigating the biochemical and physiological mechanisms underlying PMN activation.