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Updated: Apr 13, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Circulating miR-499 are novel and sensitive biomarker of acute myocardial infarction
Lizhu Zhang1, Xi Chen1, Tong Su1
11 Department of Cardiology, 2 Department of Laboratory Medicine, Wuxi Second People's Hospital of Nanjing Medical University, Wuxi 214002, China.
Background:
miRNAs are known to play a crucial role in cardiac development, and the expression of miRNA is altered in the diseased heart. The aim of this study was to investigate the value of plasma microRNA-499 (miR-499) as a novel biomarker for early diagnosis of acute myocardial infarction (AMI).
Methods:
Enrolled in this study were 227 patients with chest pain on presentation to the departments of emergency and cardiology of Wuxi Second People's Hospital between October 2011 and May 2014. Additional 100 healthy individuals who received physical examination in the same hospital during the same period were used as control. Plasma was collected at admission, and the abundance of miR-499 was measured using reverse transcriptase-polymerase chain reaction (RT-PCR).
Results:
MiR-499 was significantly elevated in 142 patients diagnosed with AMI as compared with 85 patients in non-AMI group and 100 subjects in healthy control group. Plasma miR-499 were already detectable in the plasma as early as 1 h after onset of chest pain in AMI patients, and continued to increase gradually without any sign of decreasing tendency within 9 h in AMI patients. miR-499 was highly positively correlated with the serum creatine kinase-MB (CK-MB) and cTnI. The area under the curve (AUC) of miR-499 for the diagnosis of AMI was 0.86, with an optimal cut-off value of 4.79, sensitivity of 80%, and specificity of 80.28%.
Conclusions:
miR-499 was shown to substantially increase the diagnostic accuracy of CK-MB and cTnI in the diagnosis of AMI, and therefore it may prove to be a useful marker for early diagnosis of AMI.
Insights
Plasma microRNA-499 (miR-499) shows promise as a biomarker for early acute myocardial infarction (AMI) diagnosis. Elevated miR-499 levels detected early after chest pain onset can improve diagnostic accuracy for AMI.
Area of Science:
- Cardiology
- Molecular Biology
- Biomarker Discovery
Background:
- MicroRNAs (miRNAs) are critical in cardiac development.
- Altered miRNA expression is observed in heart disease.
- Investigating plasma miR-499 as a novel biomarker for acute myocardial infarction (AMI) is crucial.
Purpose of the Study:
- To evaluate the diagnostic value of plasma miR-499 for the early detection of AMI.
- To assess the correlation of miR-499 with established cardiac biomarkers.
Main Methods:
- 227 patients with chest pain and 100 healthy controls were enrolled.
- Plasma miR-499 levels were measured using RT-PCR.
- Correlation with serum creatine kinase-MB (CK-MB) and cTnI was analyzed.
Main Results:
- Plasma miR-499 was significantly elevated in AMI patients compared to non-AMI and healthy groups.
- miR-499 was detectable within 1 hour of chest pain onset in AMI patients.
- The area under the curve (AUC) for miR-499 in AMI diagnosis was 0.86, with 80% sensitivity and 80.28% specificity.
Conclusions:
- Plasma miR-499 is a potential novel biomarker for early AMI diagnosis.
- miR-499 enhances the diagnostic accuracy of CK-MB and cTnI.
- Further validation may establish miR-499 as a valuable tool in clinical practice.
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