Reference values for reticulocyte parameters and hypochromic RBC in healthy children
C Teixeira1, J Barbot2, M I Freitas1
1Department of Pathology, Laboratory Hematology Unit, Centro Hospitalar do Porto, Porto, Portugal.
Insights
This study establishes pediatric reference values for novel reticulocyte and hypochromic red blood cell parameters using the ADVIA 2120 analyzer. These findings provide crucial data for pediatric hematology diagnostics and research.
Area of Science:
- Hematology
- Pediatrics
- Clinical Diagnostics
Background:
- Modern hematology analyzers offer new erythrocyte and reticulocyte parameters valuable in pediatric care.
- Pediatric reference ranges for these indices are currently limited.
- This study addresses the need for method-specific pediatric reference values.
Purpose of the Study:
- To establish pediatric reference values for reticulocyte parameters and hypochromic red blood cells.
- To utilize the ADVIA 2120 hematology analyzer for these measurements.
- To provide data for improved clinical practice and research in pediatrics.
Main Methods:
- Prospective study involving 311 healthy children aged 6 months to 18 years.
- Hematological parameters measured using the ADVIA 2120 analyzer.
- Participants grouped into three age cohorts; gender distribution analyzed for ages ≥12 years.
Main Results:
- Provided age- and gender-specific reference limits for reticulocyte hemoglobin content (CHr or Ret-He), Delta-He, immature reticulocyte fraction (IRF), and percentage of hypochromic red blood cells (%Hypo).
- Significant gender differences observed for Delta-He, IRF, and %Hypo in the 12-17 years group.
- CHr showed no significant gender variation across all age groups (median 30.8 ± 1.7 pg).
Conclusions:
- Establishing reliable pediatric reference intervals for novel hematological parameters is essential.
- These reference values offer valuable prospects for clinical practice in pediatrics.
- The findings support the use of these parameters in pediatric research.
Introduction:
New erythrocyte and reticulocyte parameters available on modern hematology analyzers have shown to be useful markers in children. However, pediatric method-specific reference ranges for these indices are sparse. We aim to establish pediatric reference values for reticulocyte parameters and hypochromic red blood cells in healthy children with the ADVIA 2120 hematology analyzer.
Methods:
Prospective study of 311 healthy children aged from 6 months to 18 years old with normal hematological parameters. ADVIA 2120 hematology analyzer (Siemens Healthcare Diagnostics, Amadora, Portugal) was used for whole-blood hematological measures. The sample population was grouped according to age into three cohorts, and gender distribution was used for participants aged ≥12 years.
Results:
Age- and gender-specific reference limits of reticulocyte hemoglobin content (CHr or Ret-He), difference between the reticulocyte and erythrocyte hemoglobin content (Delta-He), immature reticulocyte fraction (IRF) and percentage of hypochromic red blood cells (%Hypo) are provided. Delta-He, IRF and %Hypo showed statistically significant gender differences in the 12-17 years group. CHr presented no significant gender variation within all age groups (median 30.8 ± 1.7 pg).
Conclusion:
Establishing reliable pediatric reference intervals for these novel hematological parameters may offer valuable prospects for clinical practice and research in the pediatrics field.
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