Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

6.5K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.7K
1.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

8.0K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
8.0K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

14.1K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
14.1K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

9.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular matrix-driven patient stratification and network modeling reveal distinct molecular grades with potential clinical implications.

NPJ systems biology and applications·2026
Same author

Molecular contrastive learning with graph attention network (MoCL-GAT) for enhanced molecular representation.

BMC bioinformatics·2026
Same author

Neurodevelopmental disorders and cancer networks share pathways; but differ in mechanisms, signaling strength, and outcome.

bioRxiv : the preprint server for biology·2025
Same author

Mitochondrial one-carbon metabolism is required for TGF-β-induced glycine synthesis and fibrotic responses.

Nature communications·2025
Same author

Co-occurring Mutations in Different Genes Can Fuel Oncogenic Signaling and Serve as Metastatic Tumor Markers.

bioRxiv : the preprint server for biology·2025
Same author

Mutations in tumor signaling, metastases, and synthetic lethality establish distinct patterns.

PLoS computational biology·2025

Related Experiment Video

Updated: Apr 13, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

20.1K

The PI3K/AKT/mTOR interactive pathway.

Tulin Ersahin1, Nurcan Tuncbag, Rengul Cetin-Atalay

  • 1Cancer Systems Biology Laboratory, Graduate School of Informatics, ODTU, 06800 Ankara, Turkey. rengul@metu.edu.tr.

Molecular Biosystems
|April 30, 2015
PubMed
Summary
This summary is machine-generated.

The phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of the rapamycin (mTOR) pathway is crucial in cancer. Understanding its complex network aids in developing personalized combination therapies for improved cancer treatment.

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

930
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.5K

Related Experiment Videos

Last Updated: Apr 13, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

20.1K
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

930
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Signaling Pathways

Background:

  • The PI3K/AKT/mTOR pathway is frequently altered in various cancers, regulating key cellular functions like survival and proliferation.
  • This pathway interacts with compensatory signaling networks, notably the RAF/MEK/ERK pathway, influencing cancer progression.
  • Current targeted therapies show limited success due to tumor heterogeneity, highlighting the need for improved strategies.

Purpose of the Study:

  • To present a comprehensive network model of the PI3K/AKT/mTOR pathway.
  • To elucidate the intricate crosstalk between the PI3K/AKT/mTOR pathway and compensatory signaling networks.
  • To provide a tool for detailed study of AKT signaling and enhance personalized combinatorial therapeutic strategies.

Main Methods:

  • Network analysis of the PI3K/AKT/mTOR signaling pathway.
  • Integration of data on upstream regulators and downstream effectors.
  • Mapping of crosstalk with compensatory pathways like RAF/MEK/ERK.

Main Results:

  • A detailed map of the PI3K/AKT/mTOR network, including its interactions.
  • Identification of key crosstalk points with other signaling pathways.
  • Demonstration of the network's utility in understanding AKT signaling.

Conclusions:

  • The PI3K/AKT/mTOR pathway's complexity necessitates comprehensive network analysis.
  • Understanding pathway crosstalk is vital for overcoming therapeutic challenges.
  • This network model can guide the development of more effective personalized cancer treatments.