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Updated: Aug 12, 2026

Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
Characterization of a post-column reaction-laser-induced fluorescence detector for capillary zone electrophoresis
1Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Abstract:
Several modifications have been made to a post-column labeling system for use with capillary zone electrophoresis. Fluorescence excitation is now performed with a helium-cadmium laser rather than an arc lamp. The focusability of the laser beam allows the use of larger diameter capillaries in the post-column reactor without the excessive band broadening observed previously. These larger capillaries can be assembled in the reactor much more easily. Another improvement is that the flow-rate of the labeling reagent can now be accurately controlled and determined. Incorporating these changes, the performances of two reactors with capillaries of the same dimensions are compared.
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