Temafloxacin disk potency and tentative interpretive criteria for susceptibility tests

Area of Science:

• Microbiology
• Clinical Pharmacy
• Infectious Diseases

Background:

• Temafloxacin is an antibiotic used to treat bacterial infections.
• Accurate susceptibility testing is crucial for effective antibiotic therapy.
• Standardized disk diffusion methods are widely used for antimicrobial susceptibility testing.

Purpose of the Study:

• To evaluate the efficacy of temafloxacin disk susceptibility testing criteria.
• To determine optimal disk concentrations and zone size breakpoints for temafloxacin.
• To establish reliable methods for assessing bacterial susceptibility to temafloxacin.

Main Methods:

• Testing of 697 bacterial isolates using temafloxacin disk diffusion.
• Evaluation of both 5-microgram and 10-microgram temafloxacin disks.
• Correlation of zone sizes with Minimum Inhibitory Concentrations (MICs).

Main Results:

• Both 5- and 10-microgram temafloxacin disks were found to be satisfactory.
• Recommended breakpoints for a 5-microgram disk: resistance ≤ 12 mm (MIC > 4.0 µg/ml), susceptibility > 16 mm (MIC ≤ 2.0 µg/ml).
• These criteria allow for accurate differentiation between susceptible and resistant bacterial strains.

Conclusions:

• The 5-microgram temafloxacin disk test is a reliable method for determining bacterial susceptibility.
• Established zone size breakpoints provide clear guidelines for clinical interpretation.
• This standardized approach aids in guiding appropriate temafloxacin therapy.