Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.8K
Rab Cascades01:25

Rab Cascades

3.8K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.8K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.3K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.4K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

10.3K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
10.3K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

14.1K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ELOF1 is a core component of the promoter-proximal paused RNA polymerase II complex.

bioRxiv : the preprint server for biology·2026
Same author

A delayed translocation into the endoplasmic reticulum controls the post-translational modifications of PD-L1.

Nature communications·2026
Same author

Nanoparticle Emissions of Internal Combustion Engines: From Retrospective to Outlook.

Chimia·2026
Same author

Retrospective on the Introduction of Particle Filters to Eliminate Emissions of Nanoparticles from Engine Exhaust Gas.

Chimia·2026
Same author

Hybrid endosomal coats contain different classes of sorting nexins.

The EMBO journal·2026
Same author

Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.

eLife·2025

Related Experiment Video

Updated: Apr 13, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.1K

A modular tethering complex for endosomal recycling.

Yann Desfougères1, Massimo D'Agostino1, Andreas Mayer1

  • 1Département de Biochimie, Université de Lausanne, 1066 Epalinges, Switzerland.

Nature Cell Biology
|May 1, 2015
PubMed
Summary
This summary is machine-generated.

Researchers identified a new complex of tethering factors. This discovery sheds light on how proteins are recycled through the endolysosomal system to the cell surface.

More Related Videos

In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.7K
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

3.0K

Related Experiment Videos

Last Updated: Apr 13, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.1K
In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.7K
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

3.0K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • The endolysosomal system is crucial for protein processing and transport.
  • Mechanisms of protein trafficking within this system remain incompletely understood.
  • Efficient recycling of proteins to the cell surface is vital for cellular function.

Purpose of the Study:

  • To elucidate the molecular machinery involved in protein recycling within the endolysosomal system.
  • To identify novel factors that regulate protein transport towards the cell surface.

Main Methods:

  • Biochemical assays to identify protein complexes.
  • Cellular imaging to track protein localization and movement.
  • Genetic manipulation to assess the function of identified factors.

Main Results:

  • A novel complex of tethering factors was identified.
  • This complex plays a key role in the recycling pathway of endolysosomal proteins.
  • The identified complex facilitates protein transport towards the plasma membrane.

Conclusions:

  • The newly identified tethering factor complex represents a significant addition to our understanding of endolysosomal protein trafficking.
  • This finding provides a molecular basis for protein recycling to the cell surface.
  • Further research into this complex may reveal new therapeutic targets for diseases involving protein mis-trafficking.