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Published on: June 23, 2014
Heterogeneous Nuclear Ribonucleoprotein A2/B1 as a Target Antigen in Han Chinese for BD Patients
Jinghui Liang, Weikang Yang, Xiangyu Meng
1School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, 30 Xueyuan Rd, Beijing 100083, China. hongwudu@ustb.edu.cn.
Abstract:
Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF- TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
