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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Apr 13, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

SNES makes sense? Single-cell exome sequencing evolves.

Thierry Voet^1,2, Peter Van Loo^3,4

¹Department of Human Genetics, Laboratory of Reproductive Genomics, KU Leuven, 3000, Leuven, Belgium. Thierry.Voet@med.kuleuven.be.

|May 1, 2015

Summary

This summary is machine-generated.

New single-cell sequencing technology allows researchers to analyze all human coding DNA sequences. This breakthrough offers unprecedented detail for studying individual cells and their genomes.

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Last Updated: Apr 13, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Single-cell sequencing technologies are advancing rapidly.
Understanding cellular heterogeneity is crucial in biology and medicine.

Purpose of the Study:

To introduce a novel method for interrogating the entire human genome at single-cell resolution.
To enable comprehensive analysis of coding sequences within individual cells.

Main Methods:

Development of a new high-resolution sequencing technique.
Application of the method to analyze human genomic DNA at the single-cell level.

Main Results:

Successful interrogation of all coding sequences of the human genome.
Demonstration of the method's capability for single-cell analysis.

Conclusions:

The new technology significantly enhances the potential of single-cell genomics.
This advancement provides a powerful tool for future biological and medical research.