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Updated: Apr 13, 2026
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Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
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Direct Detection of Influenza A and B Viruses in Less Than 20 Minutes Using a Commercially Available Rapid PCR Assay
Matthew J Binnicker1, Mark J Espy2, Cole L Irish2
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA binnicker.matthew@mayo.edu.
Journal of Clinical Microbiology
|May 1, 2015
Abstract:
We compared an FDA-cleared rapid (<20 min) PCR assay (Cobas Liat; Roche Diagnostics) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focus Diagnostics) using respiratory swabs (n = 197). The Cobas Liat influenza A and B assays demonstrated sensitivities of 99.2% (123/124) and 100% (23/23), respectively, while showing a specificity of 100% for each target.
