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Updated: Apr 13, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
Published on: May 2, 2025
The hunt for origins of DNA replication in multicellular eukaryotes
John M Urban1, Michael S Foulk2, Cinzia Casella3
1Division of Biology and Medicine, Department of Molecular Biology, Cell Biology and Biochemistry, Brown University Sidney Frank Hall, 185 Meeting Street, Providence, RI 02912 USA.
Abstract:
Origins of DNA replication (ORIs) occur at defined regions in the genome. Although DNA sequence defines the position of ORIs in budding yeast, the factors for ORI specification remain elusive in metazoa. Several methods have been used recently to map ORIs in metazoan genomes with the hope that features for ORI specification might emerge. These methods are reviewed here with analysis of their advantages and shortcomings. The various factors that may influence ORI selection for initiation of DNA replication are discussed.
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