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iTRAQ-based quantitative subcellular proteomic analysis of Avibirnavirus-infected cells
Yanting Sun1, Boli Hu2, Chengfei Fan1
1Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, P. R. China.
Electrophoresis
|May 2, 2015
Summary
Infectious bursal disease virus (IBDV) alters host cell proteins, impacting immune response and cellular processes. This study identifies key host-viral interactions crucial for understanding IBDV infection mechanisms.
Area of Science:
- Virology
- Proteomics
- Cell Biology
Background:
- Infectious bursal disease virus (IBDV) utilizes host cell machinery for replication.
- Understanding host-pathogen interactions is key to controlling viral diseases.
Purpose of the Study:
- To comprehensively analyze host protein changes during early IBDV infection.
- To identify host factors involved in IBDV replication and pathogenesis.
Main Methods:
- Subcellular fractionation combined with LC-MS/MS quantitative proteomics.
- Quantitative real-time RT-PCR for gene expression analysis.
- Confocal microscopy for protein colocalization studies.
Main Results:
- IBDV infection significantly altered the abundance and localization of 3211 proteins.
- 23 cytoplasmic and 34 nuclear proteins showed significant changes.
- Key pathways affected include mTOR, PI3K/Akt, and interferon-β signaling.
- Colocalization of IBDV protein VP3 with specific host nuclear proteins was observed.
Conclusions:
- IBDV infection profoundly impacts host cell proteome and subcellular localization.
- Identified host proteins and pathways offer insights into IBDV pathogenesis.
- Findings provide a foundation for developing targeted antiviral strategies.
