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Updated: Apr 13, 2026
Antigenic Liposomes for Generation of Disease-specific Antibodies
Published on: October 25, 2018
Development of dual toxoid-loaded layersomes for complete immunostimulatory response following peroral administration
Harshad Harde1, Krupa Siddhapura, Ashish Kumar Agrawal
1Centre for Pharmaceutical Nanotechnology, Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research, SAS Nagar, Punjab 160 062, India.
Aim:
Present study reports the development of divalent vaccine with enhanced protection, permeation and presentation following peroral immunization.
Materials & Methods:
Layersomes were prepared by layer-by-layer tuning of polyelectrolytes on liposomes template. The developed system was evaluated for in vitro stability of antigen and layersomes, cell-based assays and immunization experiments in mice.
Results:
Layersomes exhibited enhanced stability in simulated biological fluids, still preserving the integrity, biological activity and conformational stability of toxoids. Layersomes also exhibited complete and protective (>0.1 IU/ml) immunostimulatory response include serum IgG titer, mucosal sIgA titer and cytokines (IL-2 and IFN-γ) levels following peroral administration.
Conclusion:
The positive findings of proposed strategy are expected to contribute significantly in the field of stable liposomes technology and peroral immunization.
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