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Updated: Apr 13, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Sensitivity improvement of kukoamine determination by complexation with dihydrogen phosphate anions in capillary zone
Yuan-Yuan Li1, Rui Di1, Wing-Leung Hsu1
1Department of Biomedical Sciences, Research Group for Bioactive Products, City University of Hong Kong, Hong Kong SAR, P. R., China.
Abstract:
A novel complexation between kukoamines and dihydrogen phosphate ions (DPI) during CZE was discovered to improve the UV signal of kukoamine by around 30-fold. This complexation formed by electric current was attributed to the hydrogen bonding of hydroxyl and amino (or amide) groups between the analyte and electrolyte anions. The established CZE method is low-cost, easy to operate, and eco-friendly, and it was shown to be superior to HPLC in terms of separation capability, efficiency, specificity, and sensitivity. We believe that our CZE method can be applied as an alternative to HPLC for kukoamine assay. The approach described here can be also extended for analyzing other compounds with similar functional groups.
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