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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
Published on: February 28, 2019
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Demonstration of simultaneous experiments using thin crystal multiplexing at the Linac Coherent Light Source
Y Feng1, R Alonso-Mori1, T R M Barends2
1Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
Journal of Synchrotron Radiation
|May 2, 2015
Summary
Multiplexing hard X-ray beams from the Linac Coherent Light Source (LCLS) was achieved using diamond crystals. This enabled simultaneous experiments, preserving beam quality for protein crystallography and XANES studies.
Area of Science:
- X-ray science
- Materials science
- Biophysics
Background:
- Advanced X-ray sources like the LCLS enable cutting-edge research.
- Simultaneous experiments can increase efficiency but require high-quality, split beams.
Purpose of the Study:
- To demonstrate hard X-ray beam multiplexing using spectral division.
- To validate the preservation of wavefront and coherence properties for simultaneous measurements.
- To showcase the feasibility of multiplexing for complex experiments.
Main Methods:
- Utilized a diamond thin-crystal monochromator in Bragg geometry for spectral division.
- Performed serial femtosecond crystallography on a protein.
- Conducted time-resolved X-ray absorption near-edge structure (XANES) studies on an iron spin-crossover system.
Main Results:
- Successfully multiplexed the LCLS hard X-ray beam.
- Preserved wavefront and coherence properties of both reflected and transmitted beams.
- Obtained comparable experimental results from multiplexed beams as from dedicated beams.
Conclusions:
- Hard X-ray beam multiplexing is feasible with high-quality beam preservation.
- Simultaneous experiments using multiplexed beams yield results of equivalent quality.
- This technique enhances experimental efficiency at X-ray free-electron lasers.

