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Immobilized laccase mediated dye decolorization and transformation pathway of azo dye acid red 27
Meenu Chhabra1, Saroj Mishra2, Trichur Ramaswamy Sreekrishnan2
1Department of Biology, Indian Institute of Technology-Jodhpur, Jodhpur, 342011 Rajasthan India.
Background:
Laccases have good potential as bioremediating agents and can be used continuously in the immobilized form like many other enzymes.
Methods:
In the present study, laccase from Cyathus bulleri was immobilized by entrapment in Poly Vinyl Alcohol (PVA) beads cross-linked with either nitrate or boric acid. Immobilized laccase was used for dye decolorization in both batch and continuous mode employing a packed bed column. The products of degradation of dye Acid Red 27 were identified by LC MS/MS analysis.
Results:
The method led to very effective (90%) laccase immobilization and also imparted significant stability to the enzyme (more than 70% after 5 months of storage at 4°C). In batch decolorization, 90-95% decolorization was achieved of the simulated dye effluent for up to 10-20 cycles. Continuous decolorization in a packed bed bioreactor led to nearly 90% decolorization for up to 5 days. The immobilized laccase was also effective in decolorization and degradation of Acid Red 27 in the presence of a mediator. Four products of degradation were identified by LC-MS/MS analysis.
Conclusions:
The immobilized laccase in PVA-nitrate was concluded to be an effective agent in treatment of textile dye effluents.
