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Updated: Apr 13, 2026
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Published on: August 6, 2018
PK study of octreotide based on LC-MS/MS combining protein precipitation and impurity extraction technique
Qian Wang1, Yan Liang, Tai Rao
1Key Lab of Drug Metabolism & Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Tongjiaxiang 24, Nanjing 210009, China.
Aim:
To establish a robust methodology for quantitative analysis of therapeutic peptide in biological samples.
Materials & Methods:
Octreotide was chosen as a model therapeutic peptide, and oxidized-octreotide was synthesized as internal standard. Protein precipitation combining liquid-liquid extraction technique was adopted to enhance the recovery and reduce the endogenous interferences effectively. A LC-MS/MS method for the quantification of octreotide in plasma has been optimized and validated according to FDA guidelines.
Results:
Linearity, selectivity, accuracy, precision, stability, matrix effect and recovery were within bioanalytical method validation acceptance criteria as FDA guidelines. The methodology was then successfully applied into the studies for octreotide.
Conclusion:
This robust methodology would be useful for the PK studies for octreotide and other therapeutic peptides.
