Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characteristics and Risk Factors of Pollen-Food Allergy Syndrome in Patients With Hay Fever: A Digital Cross-Sectional Cohort Study Using AllerSearch.

Journal of investigational allergology & clinical immunology·2025

Same author

Longitudinal whole blood transcriptome analysis of a septic shock patient with secondary hemophagocytic syndrome.

QJM : monthly journal of the Association of Physicians·2024

Same author

Intravitreal fasudil monotherapy for treatment of refractory diabetic macular edema: A prospective interventional case series.

Journal francais d'ophtalmologie·2023

Same author

Effect of Blonanserin on the Proliferation and Migration of Glioblastoma Cells.

Die Pharmazie·2023

Same author

Roth spots in gallbladder cancer.

Journal francais d'ophtalmologie·2023

Same author

Corrigendum to "Prognostic value of sequencing-based minimal residual disease detection in patients with multiple myeloma who underwent autologous stem-cell transplantation": [Annals of Oncology 28 (2017):2503-2510].

Annals of oncology : official journal of the European Society for Medical Oncology·2022

Search research articles

Related Experiment Video

Updated: Apr 13, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Photon-counting 1.0 GHz-phase-modulation fluorometer.

T Mizuno¹, S Nakao¹, Y Mizutani¹

¹Division of Energy System, Institute of Technology and Science, Tokushima University, 2-1 Minami-Jyosanjima, Tokushima 770-8506, Japan.

The Review of Scientific Instruments

|May 3, 2015

Summary

This summary is machine-generated.

We developed an advanced photon-counting phase-modulation fluorometer (PC-PMF) for high-speed fluorescence lifetime measurements. This improved system offers precise phase measurements and surpasses conventional methods in speed and accuracy.

More Related Videos

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Related Experiment Videos

Last Updated: Apr 13, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Area of Science:

Analytical Chemistry
Spectroscopy
Biophysics

Background:

Photon-counting phase-modulation fluorometry (PC-PMF) offers unique capabilities for fluorescence measurements.
Previous PC-PMF designs were limited in scope, primarily recognized for weak fluorescence detection.
The potential of PC-PMF for advanced fluorescence lifetime measurements has been recently recognized.

Purpose of the Study:

To present an improved photon-counting phase-modulation fluorometer (PC-PMF) with enhanced performance.
To highlight the significance and potential of PC-PMF for fluorescence lifetime measurements.
To demonstrate the advantages and usefulness of the improved PC-PMF through performance tests.

Main Methods:

Construction of an improved PC-PMF with a 1.0 GHz maximum modulation frequency.
Utilizing time-correlated single-photon-counting electronics for phase domain measurements.
Employing a laser diode (LD) as the excitation source and designing a stable LD driver.
Obtaining sinusoidal histogram waveforms for precise phase measurements.

Main Results:

The improved PC-PMF enables high-speed fluorescence measurements exceeding conventional detector bandwidths.
Performance comparison with conventional phase-modulation fluorometer (PMF) focused on fluorescence intensity and resolution time.
Demonstrated usefulness of the PC-PMF through fundamental performance tests.

Conclusions:

The enhanced PC-PMF is a valuable tool for precise fluorescence lifetime measurements.
The system's high-speed capability and accuracy surpass traditional methods.
The improved design opens new avenues for fluorescence spectroscopy research.