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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

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Published on: August 23, 2019

Clusterin transcript variants expression in thyroid tumor: a potential marker of malignancy?

Paolo Fuzio¹, Anna Napoli², Anna Ciampolillo³

¹Institute of Biomedical Technologies, National Research Council (CNR), Via G. Amendola, 122/D, 70126, Bari, Italy. paolo.fuzio@ba.itb.cnr.it.

|May 3, 2015

Summary

Clusterin (CLU) transcript variants show altered expression in thyroid cancer, with CLU2 increasing while CLU1 decreases. This CLU2:CLU1 ratio shift may help diagnose indeterminate thyroid nodules.

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An Ex vivo Culture System to Study Thyroid Development

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

Area of Science:

Molecular biology
Oncology
Biomarker research

Background:

Clusterin (CLU) is a multifunctional protein involved in cell proliferation and death.
CLU transcript variants play a critical role in neoplastic transformation.

Purpose of the Study:

To investigate the regulation of CLU transcript variants expression.
To assess the potential of CLU transcript variants as biomarkers for thyroid nodules.

Main Methods:

In vivo model system using neoplastic tissues and fine needle aspiration biopsy (FNAB) samples.
Immunohistochemical analyses for CLU expression.
Quantitative polymerase chain reaction (qPCR) to analyze CLU transcript variants (CLU1 and CLU2).

Main Results:

Overall CLU up-regulation observed in papillary carcinoma.
Specific increase in CLU2 transcript and decrease in CLU1 transcript in papillary carcinomas.
Increased CLU2 transcript levels in patients with histologically confirmed thyroid cancer (TIR 3).

Conclusions:

A specific alteration in the CLU2:CLU1 ratio favoring CLU2 was identified.
This finding provides evidence for CLU transcript variants as potential biomarkers.
CLU variants could aid in the accurate assessment of indeterminate thyroid nodules.