Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A HaloTag Knock-In Resource for In Vivo and In Vitro Analysis of Endogenous Polycystin-2 Localization, Turnover, and Transport.

Journal of the American Society of Nephrology : JASN·2026
Same author

Characterization and Phenotypic Assessment of Zebrafish Disease Models for Splice Modulation Studies.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

<i>p21</i>, <i>ccng1</i>, <i>foxo3b</i>, and <i>fbxw7</i> contribute to <i>p53</i>-dependent cell cycle arrest.

iScience·2025
Same author

Engineering precision zebrafish alleles of human disease.

bioRxiv : the preprint server for biology·2025
Same author

Macrophage Accumulation and Cyst Expansion in Pkd2 , Ift88 , and Double Mutant Mouse Models.

Journal of the American Society of Nephrology : JASN·2025
Same author

Disrupted development of sensory systems and the cerebellum in a zebrafish ebf3a mutant.

G3 (Bethesda, Md.)·2025

Related Experiment Video

Updated: Apr 13, 2026

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy
08:26

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Published on: October 19, 2021

6.4K

Activity Suppression Behavior Phenotype in SULT4A1 Frameshift Mutant Zebrafish.

Frank Crittenden1, Holly R Thomas1, John M Parant1

  • 1Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama (F.C., H.R.T., J.M.P., C.N.F.).

Drug Metabolism and Disposition: the Biological Fate of Chemicals
|May 3, 2015
PubMed
Summary

Sulfotransferase 4A1 (SULT4A1) gene mutations in zebrafish cause significantly reduced daytime activity. This finding reveals a novel function for SULT4A1 in regulating behavior and neural activity.

More Related Videos

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.8K
In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

21.6K

Related Experiment Videos

Last Updated: Apr 13, 2026

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy
08:26

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Published on: October 19, 2021

6.4K
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.8K
In Vivo Modeling of the Morbid Human Genome using Danio rerio
12:31

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

21.6K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Sulfotransferase (SULT) 4A1 is a highly conserved, neural-specific enzyme identified in 2000.
  • Despite its conservation, SULT4A1's function and substrate remain unknown.
  • SULT4A1 does not bind the sulfate donor 3'-phosphoadenosine-5'-phosphosulfate, unlike other SULTs.

Purpose of the Study:

  • To investigate the function of SULT4A1 in vivo.
  • To characterize the behavioral effects of SULT4A1 gene mutations in zebrafish.

Main Methods:

  • Heritable mutations in the SULT4A1 gene were generated in zebrafish using transcription activator-like effector nucleases.
  • A specific mutation, SULT4A1(Δ8), was created, resulting in a frameshift and premature stop codon.
  • EthoVision video tracking software was employed to quantify activity levels in mutant and wild-type zebrafish over a 48-hour light/dark cycle.

Main Results:

  • Zebrafish with the SULT4A1(Δ8/Δ8) mutation exhibited significantly reduced daytime activity.
  • Mutant fish displayed increased inactivity bout length and frequency compared to wild-type controls.
  • These behavioral changes represent an abnormal phenotype in the typically diurnal zebrafish.

Conclusions:

  • The SULT4A1 gene plays a crucial role in regulating daytime activity levels in zebrafish.
  • The study provides the first evidence for a functional role of SULT4A1 in vivo.
  • These findings open new avenues for understanding SULT4A1's involvement in neural function and behavior.