Related Experiment Video
Updated: Apr 13, 2026
09:39
A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
Published on: December 27, 2016
18.8K
Wound care antiseptics - performance differences against Staphylococcus aureus in biofilm
1Department of Laboratory Services, Section for Bacteriology - Aquatic and Terrestrial, Norwegian Veterinary Institute, Ullevålsveien 68, Pb 750 Sentrum, N-0106, Oslo, Norway. lene.vestby@vetinst.no.
Acta Veterinaria Scandinavica
|May 4, 2015
Summary
Iodine treatment was most effective against Staphylococcus aureus biofilms, followed by Pyrisept and chlorhexidine. This study highlights the importance of testing antiseptics on biofilm bacteria for effective wound infection treatment.
Area of Science:
- Microbiology
- Antimicrobial Efficacy
- Wound Infections
Background:
- Staphylococcus aureus is a common cause of infected wounds and a proficient biofilm producer.
- Biofilms are prevalent in chronic wounds (up to 60%) and increase bacterial tolerance to antiseptics.
- Current antiseptic testing often overlooks biofilm bacteria, potentially leading to ineffective treatments.
Purpose of the Study:
- To compare the efficacy of three antiseptic categories against Staphylococcus aureus biofilms.
- To identify the most effective antiseptic for treating S. aureus biofilm infections.
Main Methods:
- Comparative analysis of antiseptic efficacy against S. aureus biofilms.
- Evaluation of biguanide (chlorhexidine), quaternary ammonium compound (Pyrisept), and iodine/iodophores (2% iodine liniment).
Main Results:
- Significant differences in bactericidal effects against S. aureus biofilms were observed.
- Iodine treatment demonstrated the highest efficacy, followed by Pyrisept, and then chlorhexidine.
Conclusions:
- Antiseptic efficacy against S. aureus biofilms varies significantly.
- Results provide guidance for selecting effective antiseptics for biofilm-infected wounds.
- Emphasizes the need for antiseptic qualification using biofilm bacteria, not just planktonic cells.
Related Concept Videos
01:23Hand hygiene
6.7K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Hand washing...
6.7K
01:28Antimicrobial Effectiveness
2.1K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.1K
01:29Biofilms
2.0K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
2.0K
01:18Surface Membrane Barriers
3.5K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
3.5K
01:25Clinical Significance of Antibiotic Resistance
55
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
55
01:29Staphylococcal Skin Infections
91
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
91