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Mesenchymal Stem Cells01:19

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Related Experiment Video

Updated: Feb 9, 2026

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
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Epigenetic modulators promote mesenchymal stem cell phenotype switches.

Arshak R Alexanian1

  • 1Cell Reprogramming & Therapeutics LLC, W229 N1870 Westwood Drive, Waukesha, WI 53186 United States.

The International Journal of Biochemistry & Cell Biology
|May 5, 2015
PubMed
Summary

Adult stem cells exhibit plasticity, allowing transdifferentiation. This study shows human mesenchymal stem cells (hMSCs) can convert to neural and fat cells, demonstrating potential for regenerative medicine.

Keywords:
ChromatinEpigeneticsPlasticityReprogrammingTransdifferentiation

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Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Epigenetics

Background:

  • Adult stem cells may differentiate into various cell types, a phenomenon known as transdifferentiation or plasticity.
  • Epigenetic modifications, including DNA and histone covalent modifications, are crucial for cellular plasticity.
  • Previous work demonstrated conversion of human mesenchymal stem cells (hMSCs) into neural-like cells using epigenetic modifiers and neural factors.

Purpose of the Study:

  • To investigate the stability and plasticity of transdifferentiated cells.
  • To explore the potential for manipulating cell phenotype through epigenetic and signaling pathway modulation.

Main Methods:

  • Neurally induced hMSCs (NI-hMSCs) were exposed to adipocyte-inducing factors.
  • Morphological and proliferative changes were monitored.
  • Differentiation into adipocytes was confirmed via Oil Red O staining.
  • Transdifferentiated cells and purified adipocytes were re-exposed to neural induction medium.

Main Results:

  • NI-hMSCs reverted to fibroblast-like cells with regained proliferative capacity within 24-48 hours.
  • Approximately 6% of hMSCs differentiated into adipocytes after 3 weeks.
  • Re-exposure to neural induction medium successfully re-differentiated cells into neuronal-like cells.

Conclusions:

  • Cellular plasticity in hMSCs is reversible and can be manipulated.
  • Combining epigenetic modifiers with specific cell signaling pathways offers a method to control cell phenotype conversion.
  • These findings hold significant implications for regenerative medicine strategies.