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Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming
Junjun Ding1, Xin Huang1, Ningyi Shao2
1The Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Cell Stem Cell
|May 5, 2015
Summary
Tex10 is a crucial pluripotency factor that regulates super-enhancers (SEs) in embryonic stem cells (ESCs). It controls epigenetic modifications essential for cell identity, self-renewal, and reprogramming.
Area of Science:
- Molecular Biology
- Epigenetics
- Stem Cell Biology
Background:
- Super-enhancers (SEs) are critical regulatory elements driving cell identity genes.
- Embryonic stem cells (ESCs) rely on core pluripotency factors like Oct4, Sox2, and Nanog at SEs.
Purpose of the Study:
- To investigate the molecular mechanisms controlling SE activity in pluripotency and reprogramming.
- To identify novel factors involved in SE regulation within the pluripotency network.
Main Methods:
- Analysis of protein interaction networks centered around Sox2.
- Functional characterization of identified factors in ESCs and during reprogramming.
- Assessment of epigenetic modifications (histone acetylation, DNA demethylation) at SEs.
Main Results:
- Tex10 was identified as a key pluripotency factor interacting with Sox2.
- Tex10 is enriched at SEs in a Sox2-dependent manner.
- Tex10 coordinates histone acetylation and DNA demethylation at SEs, impacting ESC self-renewal, development, and reprogramming efficiency in human cells.
Conclusions:
- Tex10 is a core component of the pluripotency network.
- Tex10 plays a significant role in the epigenetic control of SE activity for cell fate determination.
- Tex10 is essential for maintaining pluripotency and facilitating reprogramming in both mouse and human cells.
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