Immunoblotting conditions for small peptides from streptococci
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Lauren Davey1, Scott A Halperin2, Song F Lee3
1Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada; Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, Halifax, NS B3K 6R8, Canada.
Journal of Microbiological Methods
|May 5, 2015
Abstract:
Streptococci secrete small peptides with important biological functions. These peptides are not amenable to standard immunoblotting, and are often detected indirectly using activity assays, or by alternative approaches that may be expensive and laborious. Here we describe an immunoblotting method that enables reproducible detection of these small streptococcal peptides.

