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High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus species
Raffael C Inglin1, Marc J A Stevens1, Lukas Meile1
1Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092 Zurich, Switzerland.
Journal of Microbiological Methods
|May 5, 2015
Abstract:
We describe high-throughput screening techniques to rapidly detect either antimicrobial activity, using an agar-well diffusion assay in microtiter plates, or antifungal activity using an agar-spot assay in 24-well plates. 504 Lactobacillus isolates were screened with minimal laboratory equipment and screening rates of 2000-5000 individual antimicrobial interactions.

