Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.7K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.7K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.8K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Covalent functionalization of polymeric nanoparticles prepared from nano-emulsion templates for gene silencing and antioxidant delivery at neuronal level.

International journal of pharmaceutics·2026
Same author

G-quadruplexes as therapeutic platforms for anticancer drug delivery: from intrinsic cytotoxicity to drug delivery and nanotechnology.

Expert opinion on drug delivery·2026
Same author

Engineering G-quadruplex aptamer-5-fluoro-2'-deoxyuridine conjugates: Impact of tail orientation and RNA spacer insertion on structure and antitumor activity.

International journal of biological macromolecules·2026
Same author

Enhanced muscle uptake of chemically optimized miR-23b antisense oligonucleotides as lead compounds for myotonic dystrophy type 1.

American journal of human genetics·2026
Same author

Conjugation strategy shapes antitumor efficacy and enables dose-sparing in non-antibody protein nanoconjugates.

Materials today. Bio·2026
Same author

PET-Driven Fluorescence Modulation in Halochromic Styryl Hemicyanine Dyes Targeting DNA Minor Groove.

Molecules (Basel, Switzerland)·2025

Related Experiment Video

Updated: Apr 13, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K

Variable-temperature size exclusion chromatography for the study of the structural changes in g-quadruplex.

Sanae Benabou1, Ramon Eritja2, Raimundo Gargallo1

  • 1Solution Equilibria and Chemometrics Group (Associate Unit UB-CSIC), Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028 Barcelona, Spain.

ISRN Biochemistry
|May 5, 2015
PubMed
Summary

Guanine-rich sequences in the human c-kit oncogene promoter form G-quadruplex structures. Mutations alter these structures from parallel to antiparallel G-quadruplexes, affecting their stability and form.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.8K
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

5.6K

Related Experiment Videos

Last Updated: Apr 13, 2026

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

2.0K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.8K
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

5.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Guanine-rich sequences are crucial in gene regulation.
  • The human c-kit oncogene promoter contains a G-rich region with potential for non-canonical DNA structures.

Purpose of the Study:

  • To investigate the conformational equilibria of a G-rich sequence from the human c-kit oncogene promoter.
  • To understand how mutations affect G-quadruplex formation and stability.

Main Methods:

  • Circular dichroism (CD) spectroscopy to analyze DNA conformation.
  • Variable-temperature size exclusion chromatography (SEC) to assess G-quadruplex aggregation state.

Main Results:

  • The wild-type ckit21 sequence exists as a mixture of monomeric and multimeric G-quadruplexes.
  • Mutations induced a shift from parallel to antiparallel G-quadruplex conformations.
  • Mutations also led to the disappearance of multimeric species.
  • Temperature-induced conformational shifts between antiparallel and parallel G-quadruplexes were observed in both CD and SEC data.

Conclusions:

  • The G-rich sequence in the c-kit oncogene promoter can form diverse G-quadruplex structures.
  • Specific base mutations can precisely control G-quadruplex topology and aggregation.
  • These findings provide insights into the role of G-quadruplexes in gene regulation and oncogene function.