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Related Experiment Video

Updated: Apr 13, 2026

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
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Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

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Cell chemotaxis on paper for diagnostics.

David I Walsh1, Mark L Lalli1, Juliette M Kassas1

  • 1†Department of Bioengineering, ‡Department of Chemical Engineering, §Department of Biology, ∥Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, United States.

Analytical Chemistry
|May 5, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost paperfluidic device for rapid chemokine gradient generation, enabling faster cell migration studies. The platform facilitates directed T-cell migration, paving the way for point-of-care diagnostic tools.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Traditional microfluidic chemotaxis platforms require specialized equipment and long incubation times.
  • Existing systems are not readily adaptable for rapid diagnostics or point-of-care applications.

Purpose of the Study:

  • To develop a low-cost, rapidly assembled paperfluidic device for generating stable chemokine gradients.
  • To enable short-term cell migration studies and assess directed cell movement.
  • To establish a foundational technology for future microfluidic diagnostic platforms.

Main Methods:

  • A novel paperfluidic device was designed for rapid (<1 second) chemokine gradient generation.
  • The device produces a quasi-stable gradient for at least 20 minutes.
  • Human pan-T cells were utilized to demonstrate proof-of-concept cell migration in response to the gradient.

Main Results:

  • The paperfluidic device successfully generated a sharp chemokine gradient.
  • Human pan-T cells exhibited significant directed migration towards the chemokine gradient (p ≪ 0.01).
  • Cell migration response was observed within a short 20-minute timeframe.

Conclusions:

  • The developed paperfluidic device offers a low-cost, efficient alternative for creating chemokine gradients.
  • This technology significantly reduces the time required for cell migration studies.
  • The platform represents a foundational step towards developing microfluidic chemotaxis systems for point-of-care diagnostics.