Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

131
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
131
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

127
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
127
Modified-Release Drug Delivery Systems: Stimuli-Activated01:30

Modified-Release Drug Delivery Systems: Stimuli-Activated

139
Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also...
139
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

5.0K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combining Anti-Inflammatory Drugs and Cell-Specific Expression-Suppression Reduces the Adjuvant Activity of mRNA-LNPs.

Molecular pharmaceutics·2026
Same author

Lipid nanoparticle-encapsulated microRNA-192: An anti-inflammatory adjuvant that enhances vaccine efficacy in aged mice.

Molecular therapy. Nucleic acids·2025
Same author

Lipid nanoparticle-mediated mRNA delivery system into preimplantation embryos†.

Biology of reproduction·2025
Same author

Combination of ionizable lipids with oleic acid and vitamin E scaffolds for RNA cancer vaccine delivery.

Journal of controlled release : official journal of the Controlled Release Society·2025
Same author

Neoantigen mRNA vaccines induce progenitor-exhausted T cells that support anti-PD-1 therapy in gastric cancer with peritoneal metastasis.

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·2025
Same author

Modulating Immunogenicity and Reactogenicity in mRNA-Lipid Nanoparticle Vaccines through Lipid Component Optimization.

ACS nano·2025

Related Experiment Video

Updated: Apr 13, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.3K

Multifunctional Envelope-Type Nano Device: Evolution from Nonselective to Active Targeting System.

Yasuhiro Hayashi1, Hiroto Hatakeyama1, Kazuaki Kajimoto1

  • 1Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan 060-0812.

Bioconjugate Chemistry
|May 5, 2015
PubMed
Summary

Advancements in drug delivery systems (DDS) focus on intracellular and active targeting for nucleic acids like siRNA/pDNA. Novel nanodevices, including multifunctional envelope-type nano devices (MENDs), enhance endosomal escape and enable targeted delivery for therapeutic effects.

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.5K
Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
08:14

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Published on: October 26, 2018

9.0K

Related Experiment Videos

Last Updated: Apr 13, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

15.3K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.5K
Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting
08:14

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Published on: October 26, 2018

9.0K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Pharmacology

Background:

  • Drug delivery systems (DDS) are evolving towards intracellular and active targeting strategies.
  • Efficient endosomal escape is crucial for nucleic acid delivery (siRNA/pDNA) via endocytosis.
  • Targeting specific vasculature (tumor, adipose, lung) presents challenges due to endothelial barriers.

Purpose of the Study:

  • To develop advanced nanocarriers for controlled intracellular trafficking of nucleic acids.
  • To enhance active targeting of nanoparticles to specific tissues using dual-ligand systems.
  • To create novel ligands for targeting tumor vasculature.

Main Methods:

  • Development of multifunctional envelope-type nano devices (MENDs) for siRNA/pDNA delivery.
  • Modification of MENDs with octaarginine (R8) for enhanced cellular uptake and endosomal escape.
  • Design of dual-ligand systems combining selective and cell-penetrating ligands for active targeting.
  • Creation of prohibitin-targeted nanoparticles (PTNP) for adipose tissue vasculature targeting.
  • Utilizing GALA peptide for lung endothelial cell targeting and enhanced endosomal escape.

Main Results:

  • Octaarginine-modified MENDs demonstrate control over intracellular trafficking of nucleic acid-loaded nanocarriers.
  • Dual-ligand systems show synergistic effects, enhancing active targeting capabilities.
  • PTNP effectively targets adipose endothelial cells, delivering therapeutic agents.
  • GALA peptide facilitates lung endothelial cell targeting and improves endosomal escape.
  • Novel ligands developed for tumor vasculature targeting show promise for in vivo applications.

Conclusions:

  • Progress in DDS, particularly intracellular and active targeting nanodevices, is expanding the field of nanomedicine.
  • Engineered nanocarriers like MENDs and dual-ligand systems offer improved therapeutic efficacy for nucleic acid delivery.
  • Targeted delivery to specific vasculature, including tumor and adipose tissue, is achievable with advanced DDS strategies.
  • These advancements hold significant potential for developing breakthrough nanomedicine technologies.