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Development of EV71 virus-like particle purification processes.

Shih-Yeh Lin1, Hsin-Yi Chiu1, Bor-Luen Chiang2

  • 1Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan.

Vaccine
|May 6, 2015
PubMed
Summary

This study developed an efficient production and purification process for Enterovirus 71 (EV71) virus-like particles (VLPs). The new chromatography-based method yields high-purity EV71 VLPs, advancing vaccine development.

Keywords:
ChromatographyDownstream processingEnterovirus 71PurificationVaccineVirus-like particle

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Area of Science:

  • Virology
  • Biotechnology
  • Vaccine Development

Background:

  • Enterovirus 71 (EV71) causes severe hand-foot-and-mouth disease outbreaks, particularly in young children.
  • EV71 virus-like particles (VLPs), empty viral capsids, show promise as vaccine candidates due to their immunogenicity.
  • A scalable and efficient production and purification process for EV71 VLPs is currently lacking.

Purpose of the Study:

  • To develop a scalable production and chromatography-based purification process for Enterovirus 71 (EV71) virus-like particles (VLPs).
  • To optimize EV71 VLP recovery and purity for potential clinical applications.

Main Methods:

  • EV71 VLPs were produced in High Five™ cells using a recombinant baculovirus expression system in a stirred-tank bioreactor.
  • Tangential flow filtration (TFF) was used for VLP concentration and diafiltration, removing over 80% of host cell proteins.
  • Hydroxyapatite chromatography (HAC) and size-exclusion chromatography (SEC) were employed for VLP purification, followed by final TFF concentration.

Main Results:

  • The integrated purification process achieved an overall VLP recovery of approximately 36% and a purity of about 83%.
  • The purification efficiency, in terms of recovery and purity, was comparable or superior to methods used for live EV71 virus particles.
  • The process demonstrated successful VLP production and effective removal of impurities.

Conclusions:

  • The developed production and purification strategy provides an efficient and scalable method for generating high-purity EV71 VLPs.
  • This advancement brings EV71 VLP-based vaccine candidates closer to clinical application.
  • The study highlights the potential of chromatography-based purification for viral particle-based vaccine production.