Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same author

Sequencing Ablation and Systemic Therapy in Colorectal Liver Oligometastases: An Upfront versus Delayed Approach Nationwide Analysis.

Radiology·2026
Same author

HA thermostability mutations S84F, G167N, and D168N potentiate H9N2 virus transmission in a warming environment.

Emerging microbes & infections·2026
Same author

RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation.

iScience·2026
Same author

AirNet: A Deep Learning-Driven Auto Baseline Correction Algorithm Balancing Global Smoothness and Local Fidelity.

Analytical chemistry·2026
Same author

Efficient large-fragment isogenic sequence replacement in rice via prime editing with engineered reverse transcriptase variants.

Journal of advanced research·2026

Related Experiment Video

Updated: Apr 13, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
09:51

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

16.1K

Reliable Quantitative SERS Analysis Facilitated by Core-Shell Nanoparticles with Embedded Internal Standards.

Wei Shen1, Xuan Lin2, Chaoyang Jiang3

  • 1State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), MOE laboratory of Spectrochemical Analysis and Instrumentation, The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China) http://bren.xmu.edu.cn.

Angewandte Chemie (International Ed. in English)
|May 6, 2015
PubMed
Summary
This summary is machine-generated.

Quantitative surface-enhanced Raman scattering (SERS) analysis is improved using core-molecule-shell nanoparticles. An embedded Raman probe corrects for fluctuations, enabling reliable quantification of target molecules across wide concentration ranges.

Keywords:
Raman spectroscopyanalytical methodsinternal standardsnanoparticlesquantitative analysis

More Related Videos

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.8K

Related Experiment Videos

Last Updated: Apr 13, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
09:51

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

16.1K
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
07:54

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Published on: October 27, 2020

5.0K
Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.8K

Area of Science:

  • Nanotechnology
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Quantitative analysis using surface-enhanced Raman scattering (SERS) presents significant challenges due to signal fluctuations.
  • Conventional SERS methods struggle to achieve reliable quantification over broad concentration ranges.

Purpose of the Study:

  • To develop a novel nanoparticle design for accurate and robust quantitative SERS analysis.
  • To introduce an internal standard within the nanoparticle structure for real-time signal correction.

Main Methods:

  • Design and synthesis of core-molecule-shell nanoparticles incorporating a framework molecule and a Raman probe.
  • Utilizing the Raman probe signal to compensate for variations in sample and measurement conditions.
  • Adsorption of target molecules with varying affinities onto the nanoparticle shell.

Main Results:

  • Demonstrated successful quantitative analysis of target molecules using the designed nanoparticles.
  • Achieved a linear relationship between relative SERS intensity and surface coverage over a large concentration range.
  • The embedded Raman probe effectively corrected for fluctuations, enhancing analytical reliability.

Conclusions:

  • Core-molecule-shell nanoparticles with embedded Raman probes offer a powerful strategy for quantitative SERS.
  • This approach overcomes limitations of conventional SERS, enabling precise analysis of analytes with diverse affinities.
  • The method provides a reliable platform for quantitative SERS applications previously unattainable.