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Updated: Apr 13, 2026

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
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Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

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Molecular characterization of outer membrane vesicles released from Acinetobacter radioresistens and their potential

Shweta Fulsundar1, Heramb M Kulkarni2, Medicharla V Jagannadham2

  • 1Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, India.

Microbial Pathogenesis
|May 6, 2015
PubMed

Insights

Clinical Acinetobacter radioresistens releases outer membrane vesicles (OMVs). These OMVs contain proteins involved in biofilm formation and virulence, suggesting a role in bacterial pathogenesis and host cell apoptosis.

Area of Science:

  • Microbiology
  • Bacterial pathogenesis

Background:

  • Acinetobacter radioresistens is a clinically significant bacterium.
  • Outer membrane vesicles (OMVs) are increasingly recognized as key mediators of bacterial interactions.

Purpose of the Study:

  • To characterize the outer membrane vesicles (OMVs) released by a clinical isolate of Acinetobacter radioresistens.
  • To identify proteins within these OMVs and elucidate their potential functions.

Main Methods:

  • In vitro culture of Acinetobacter radioresistens.
  • Dynamic light scattering (DLS) for OMV size determination.
  • Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for OMV proteomic analysis.

Main Results:

  • Acinetobacter radioresistens released OMVs with diameters ranging from 10 to 150 nm.
  • Proteomic analysis identified 71 proteins in the OMVs, originating from various cellular locations.
  • Identified proteins were associated with functions including biofilm formation, quorum sensing, oxidative stress tolerance, and cytotoxicity.

Conclusions:

  • Acinetobacter radioresistens actively releases OMVs.
  • OMVs from this bacterium carry proteins implicated in virulence and biofilm augmentation.
  • These OMVs may contribute to Acinetobacter radioresistens pathogenesis by inducing apoptosis.
Keywords:
Acinetobacter radioresistensApoptosisBiofilm augmentationCytotoxicityOMV

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