Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Proteasome Structure01:17

The Proteasome Structure

2.3K
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
2.3K
The Proteasome02:18

The Proteasome

10.7K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.7K
The Proteasome01:13

The Proteasome

2.0K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
2.0K
The Proteasome02:18

The Proteasome

5.1K
5.1K
Caspases01:24

Caspases

14.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
14.6K
Ribozymes02:47

Ribozymes

13.8K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "β-Triketones from Leptospermum scoparium (mānuka) oil show potential as scabicides" [Phytomedicine Volume 136, January 2025, 156321].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

In vitro assessment of a combination therapy of abametapir and flavesone as a novel topical single-dose scabies treatment.

Journal of the American Academy of Dermatology·2026
Same author

Real-time imaging on a microwell device allows first insights into the embryonic development of the ubiquitous and notorious scabies parasite.

Scientific reports·2025
Same author

Expression profiles of itch markers during scabies infection in a porcine scabies model.

PLoS neglected tropical diseases·2025
Same author

Inflammatory Responses Potentiate GAS M Protein Induced Cardiac Damage in an Experimental Model of Rheumatic Heart Disease.

Immunity, inflammation and disease·2025
Same author

β-Triketones from Leptospermum scoparium (mānuka) oil show potential as scabicides.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2025
Same journal

Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

The Biochemical journal·2026
Same journal

Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

The Biochemical journal·2026
Same journal

Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

The Biochemical journal·2026
Same journal

Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

The Biochemical journal·2026
Same journal

Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

The Biochemical journal·2026
Same journal

A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

The Biochemical journal·2026
See all related articles

Related Experiment Video

Updated: Apr 13, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.6K

Pseudoproteases: mechanisms and function.

Simone L Reynolds1, Katja Fischer1

  • 1*Infectious Diseases Department, QIMR Berghofer Medical Research Institute, Locked Bag 2000, Royal Brisbane Hospital, Brisbane, QLD 4029, Australia.

The Biochemical Journal
|May 6, 2015
PubMed
Summary
This summary is machine-generated.

Inactive enzymes, or pseudoenzymes, are increasingly recognized for their crucial roles beyond catalysis, including regulation and immune modulation. Their expanding functions and therapeutic potential are highlighted in this review.

More Related Videos

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

17.1K

Related Experiment Videos

Last Updated: Apr 13, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.6K
Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.6K
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

17.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Inactive enzymes, also termed pseudoenzymes or protease homologues, were traditionally thought to be mere regulators of active enzymes.
  • Recent characterizations reveal diverse and significant biological functions beyond simple regulation.

Purpose of the Study:

  • To review the classification, structure, function, and mechanisms of inactive enzymes.
  • To discuss the expanding roles and therapeutic potential of these molecules.

Main Methods:

  • Literature review focusing on characterized inactive enzymes.
  • Analysis of structural and functional data from emerging genomic studies.

Main Results:

  • Inactive enzymes possess a broad range of functions, including regulation, inhibition, and immune modulation.
  • New inactive enzymes are continually identified through genomic research, underscoring their abundance.

Conclusions:

  • Pseudoenzymes have critical, multifaceted roles in biological systems.
  • Their unique functions present significant opportunities for therapeutic target development.