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Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
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Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation
O Chumphukam1, T T Le, S Piletsky
1Department of Chemistry, Imperial College London, UK. t.cass@imperial.ac.uk.
Chemical Communications (Cambridge, England)
|May 6, 2015
Abstract:
The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site ('sandwich') assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.
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