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First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil
Monally C C Aquino1, Giovanni Widmer2, Anaiza S Zucatto1
1UNESP, Faculdade de Medicina Veterinária de Araçatuba, Universidade Estadual Paulista Júlio de Mesquita Filho, Araçatuba, São Paulo, Brazil.
Abstract:
With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.
