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Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

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Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

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Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2.2K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

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Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
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Related Experiment Video

Updated: Apr 13, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
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Fully resolved NMR correlation spectroscopy.

Daisy Pitoux1, Bertrand Plainchont1, Denis Merlet1

  • 1Equipe de RMN en milieu orienté Université Paris-Sud, ICMMO, UMR 8182 (CNRS-UPS), 91405 Orsay cedex (France) http://www.icmmo.u-psud.fr.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|May 6, 2015
PubMed
Summary
This summary is machine-generated.

A novel pulse sequence, push-G-SERF, enhances nuclear magnetic resonance (NMR) spectroscopy for complex molecules. This method simplifies structural analysis by providing clear spectral resolution and coupling network information around protons.

Keywords:
NMR spectroscopyoligosaccharidespure shiftselective refocusingstructure elucidation

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Organic Chemistry
  • Structural Elucidation

Background:

  • Overcrowded NMR spectra pose significant challenges for accurate structural analysis of complex organic molecules.
  • Existing NMR techniques may lack the resolution or sensitivity required for detailed analysis of specific proton environments.

Purpose of the Study:

  • To introduce and validate a new NMR pulse sequence, termed push-G-SERF.
  • To demonstrate the utility of push-G-SERF for efficient structural analysis, particularly in cases of spectral congestion.

Main Methods:

  • Development and implementation of the push-G-SERF pulse sequence.
  • Acquisition and analysis of phased 2D NMR spectra.
  • Testing the sequence's robustness on compounds with varying structural and spectral complexity using advanced NMR spectrometers.

Main Results:

  • The push-G-SERF experiment successfully separates chemical shift information (direct dimension) from scalar coupling information (indirect dimension) involving selected protons.
  • The method provides full spectral resolution in both dimensions, enabling straightforward assignment of the coupling network around specific protons.
  • Robust performance was confirmed across a range of molecular structures and spectral complexities.

Conclusions:

  • The push-G-SERF pulse sequence offers an efficient solution for structural analysis of molecules with overcrowded NMR spectra.
  • It facilitates the detailed mapping of proton-centric coupling networks, aiding in molecular structure determination.
  • This technique is valuable for synthetic and analytical chemists requiring precise structural insights.