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Related Experiment Videos

A method to quantify and correct for edge leaks in Ussing chambers.

G Kottra1, G Weber, E Frömter

  • 1Zentrum der Physiologie, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt, Federal Republic of Germany.

Pflugers Archiv : European Journal of Physiology
|November 1, 1989
PubMed
Summary

This study introduces a novel method to detect and quantify edge leaks in Ussing chambers. The technique accurately measures transepithelial resistance even in leaky chambers, distinguishing between tissue and chamber issues.

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Area of Science:

  • Physiology
  • Biophysics
  • Biotechnology

Background:

  • Ussing chambers are crucial for studying epithelial tissues.
  • Edge leaks in Ussing chambers can lead to inaccurate transepithelial resistance measurements.
  • Differentiating between leaky tissues and leaky chambers is essential for reliable results.

Purpose of the Study:

  • To develop and validate a technique for detecting and quantifying edge leaks in Ussing chambers.
  • To enable accurate transepithelial resistance measurements in the presence of edge leaks.
  • To distinguish between leaky tight junctions and leaky chambers.

Main Methods:

  • Utilizing differences in alternating current (AC) behavior at varying frequencies (low vs. high) to probe electrical field distribution.

Related Experiment Videos

  • Measuring current density at the chamber center under low and high AC frequencies.
  • Applying correction factors based on electrical field geometry, chamber dimensions, and estimated tissue resistance.

    • Main Results:

    • The presented technique successfully detects and quantifies edge leaks in Ussing chambers.
    • Accurate transepithelial resistance values can be obtained from measurements taken in leaky chambers.
    • The method allows differentiation between leaky tight junctions and leaky chambers.

    Conclusions:

    • This technique provides a reliable method to assess Ussing chamber integrity.
    • It enhances the accuracy of epithelial barrier function studies by correcting for edge leaks.
    • Researchers can now confidently determine the source of resistance discrepancies.