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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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A low cost design and fabrication method for developing a leak proof paper based microfluidic device with customized

Ankana Kakoti1, Mohd Farhan Siddiqui1, Pranab Goswami1

  • 1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati 781039, Assam, India.

Biomicrofluidics
|May 7, 2015
PubMed
Summary

This study presents a novel, leak-proof paper microfluidic device fabricated using photolithography. The design minimizes sample volume and allows easy integration of external test zones for cost-effective diagnostics.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Paper-based microfluidic devices offer low-cost diagnostics but often suffer from leakage and limited integration capabilities.
  • Existing designs may require larger sample volumes and lack flexibility for incorporating external test zones.

Purpose of the Study:

  • To develop a leak-proof paper-based microfluidic device with a novel design for integrating external test zones.
  • To reduce sample volume requirements and optimize reaction times for improved diagnostic performance.

Main Methods:

  • Fabrication using negative photolithography with a partial photoresist layer to prevent sample leakage.
  • Design incorporating channel protrusions for seamless integration of external test zones.
  • Validation using microscopy, Field Emission Scanning Electron Microscopy, and colorimetric assays.

Main Results:

  • The photoresist layer effectively blocked sample leakage and reduced required sample volume to 7 ± 0.2 μl.
  • The integrated external test zone design facilitated analyte-reagent interaction, extending reaction time.
  • Colorimetric assays demonstrated the device's efficacy for protein and acid/base detection.

Conclusions:

  • The developed paper microfluidic device is leak-proof, requires minimal sample volume, and is cost-effective (~$0.03 per device).
  • The novel design enables facile integration of customized external test zones, enhancing diagnostic versatility.
  • This technology holds potential for improved point-of-care diagnostics and analyte detection.