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Updated: Apr 13, 2026

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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Surfactant-Induced Artifacts during Proteomic Sample Preparation.

Yuhuan Ji, Minjing Liu, Markus M Bachschmid

    Analytical Chemistry
    |May 7, 2015
    PubMed
    Summary
    This summary is machine-generated.

    ProteaseMAX can cause artifactual cysteine modifications in proteomics studies. Researchers should avoid this surfactant when investigating lipid modifications and quantitative cysteine analysis.

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    Area of Science:

    • Proteomics and Mass Spectrometry
    • Biochemistry
    • Post-Translational Modifications

    Background:

    • Bottom-up proteomics is vital for identifying protein post-translational modifications (PTMs) at the peptide level using mass spectrometry (MS).
    • Enzymatic digestion, a key step in bottom-up proteomics, can introduce artifactual modifications during sample processing.
    • Investigating PTMs requires careful consideration of reagents to avoid experimental artifacts.

    Purpose of the Study:

    • To investigate potential artifactual modifications introduced during bottom-up proteomics sample preparation.
    • To identify the source of unexpected modifications observed during a study of regulator of G-protein signaling 4 PTMs.
    • To assess the impact of ProteaseMAX on cysteine residue modifications.

    Main Methods:

    • Mass spectrometry (MS) based proteomics analysis.
    • Bottom-up proteomics workflow including protein digestion.
    • Characterization of post-translational modifications on cysteine residues.

    Main Results:

    • ProteaseMAX, an acid-labile surfactant, induced in vitro hydrophobic modifications on cysteine residues.
    • These artifactual modifications mimicked S-palmitoylation and hydroxyfarnesylation.
    • The modifications targeted the cysteine thiol group, impacting quantitative accuracy.

    Conclusions:

    • ProteaseMAX should not be used in studies focusing on lipid modifications of proteins.
    • Artifactual cysteine modifications from ProteaseMAX can lead to inaccuracies in quantitative analysis of cysteine PTMs.
    • Careful reagent selection is crucial in proteomics to ensure accurate PTM characterization.