Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-β1 in renal tubular cells

The objective of our study is to investigate the effect of triptolide on expression of thrombospondin-1 and transforming growth factor β1 in renal tubular cells. Human renal tubular epithelial cells were stimulated by different concentrations of triptolide (0.1, 1, and 10 μg/L) in the presence of angiotensin-II (10(-7)mol/L). Real Time PCR was used to detect the mRNA expression of thrombospondin-1 and transforming growth factor β1. Western blot analysis was used to detect the protein expression. ELISA was used to detect the level of total and active transforming growth factor β1. The mRNA expression of thrombospondin-1 (3.66 ± 0.48 vs. 1.33 ± 0.26, p < 0.05) and transforming growth factor β1 (3.58 ± 0.59 vs. 1.26 ± 0.28, p < 0.05) were up-regulated obviously when stimulated by angiotensin-II. And the protein expression of thrombospondin-1 (0.5126 ± 0.0936 vs. 0.1025 ± 0.0761, p < 0.01) and transforming growth factor β1 (0.5948 ± 0.0736 vs. 0.1318 ± 0.0614, p < 0.01) were also up-regulated simultaneously when stimulated by angiotensin-II. The expression of thrombospondin-1 and transforming growth factor β1 induced by angiotensin-II were down-regulated markedly with 1 μg/L and 10 μg/L of triptolide in mRNA and protein levels (p < 0.05, p <  0.01). And triptolide (1 and 10 μg/L) could reduce the expression of total and active transforming growth factor β1 (p < 0.05, p < 0.01). In conclusion, triptolide can inhibit the expression of thrombospondin-1 and transforming growth factor β1 in mRNA and protein levels and down-regulate the levels of total and active transforming growth factor β1.