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Surface charge determines the lung inflammogenicity: A study with polystyrene nanoparticles.

Jeongeun Kim1, Sunay V Chankeshwara2, Frank Thielbeer2

  • 1a Lab of Toxicology, Department of Medicinal Biotechnology, College of Natural Resources and Life Science , Dong-A University , Busan , Republic of Korea .

Nanotoxicology
|May 7, 2015
PubMed
Summary

Surface charge of functionalized nanoparticles (F-PLNPs) significantly impacts lung inflammation. Zeta potential strongly correlates with inflammatory markers, validating the surface charge paradigm for predicting nanoparticle toxicity.

Keywords:
Acute lung inflammationfunctional groupparticle pathogenicity paradigmpharyngeal aspirationsurface charge paradigm

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Toxicology

Background:

  • Surface functionalization of nanoparticles (NPs) is crucial for modulating their behavior.
  • Surface properties, particularly surface charge, can influence NP toxicity.
  • Understanding NP-induced inflammation is vital for safety assessment.

Purpose of the Study:

  • To investigate the role of surface charge in acute lung inflammation and localization of functionalized polymeric NPs (F-PLNPs).
  • To synthesize and characterize a library of F-PLNPs with varying surface charges.
  • To establish a correlation between NP surface charge and inflammatory responses in the lung.

Main Methods:

  • Synthesis of eight types of F-PLNPs with diverse surface charges (zeta potentials from -41.2 to 31.0 mV) from aminated-F-PLNP.
  • Characterization of F-PLNPs for primary and hydrodynamic sizes.
  • Pharyngeal aspiration of F-PLNPs into rats to assess acute lung inflammation.
  • Measurement of inflammatory parameters including polymorphonuclear leukocytes and cytokine levels (IL-1β, CINC-3).

Main Results:

  • F-PLNPs exhibited diverse zeta potentials while maintaining identical primary particle sizes.
  • Pharyngeal aspiration led to varied acute lung inflammation responses.
  • Strong correlations were observed between F-PLNP zeta potential and key inflammation parameters: polymorphonuclear leukocytes (R²=0.90), IL-1β (R²=0.83), and CINC-3 (R²=0.86).

Conclusions:

  • Surface charge is a critical determinant of lung inflammation induced by functionalized polymeric NPs.
  • The findings confirm and extend the surface charge paradigm to functionalized NPs.
  • The surface charge paradigm serves as a valuable predictive tool for NP toxicity.