Related Experiment Video
Updated: Apr 13, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
A study on liposomal encapsulation of a lipophilic prodrug of LHRH
Rosario Pignatello1, Teresa Musumeci1, Adriana C E Graziano2
1a Section of Pharmaceutical Technology, Department of Drug Sciences and.
Abstract:
This study aimed at evaluating whether derivatization of luteinizing hormone-releasing hormone (LHRH) peptide with an amphiphilic lipoamino acid moiety could allow, along with other technological and/or pharmacokinetic advantages, to improve its encapsulation in liposomes, potentially driving its further body distribution and cellular uptake. Experimental data confirmed that a lipophilic derivative of LHRH was efficiently incorporated in various liposomal systems, differing in lipid composition and surface charge, and obtained using different methods of production. Incubation of liposomes, loaded with a fluorescent derivative of the LHRH prodrug, with NCTC keratinocytes or Caco-2 cell cultures showed that the carriers can be rapidly internalized. Conversely, the internalization of the free prodrug occurred only at very high concentrations.
Related Concept Videos
Receptor-mediated Endocytosis
Prodrugs
Prodrugs help overcome...
Bioavailability Enhancement: Drug Permeability Enhancement
Drug Biotransformation: Overview
Modified-Release Drug Delivery Systems: Site-Targeted

