Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

10.8K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
10.8K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.9K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.9K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.1K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PolyDeep Advance 3: Randomized Clinical Trial comparing PolyDeep-assisted and conventional colonoscopy for adenoma detection rate in a colorectal cancer screening program.

Endoscopy·2026
Same author

Updates and validation of the Compi RNA-seq pipeline with a case study in Alzheimer's disease.

Journal of integrative bioinformatics·2026
Same author

PIBAdb: a public cohort of multimodal colonoscopy videos and images including polyps with histological information.

Computer methods and programs in biomedicine·2026
Same author

Clinical Evaluation of PolyDeep, A Computer-Aided Detection System: A Multicenter Randomized Tandem Colonoscopy Trial.

Diagnostics (Basel, Switzerland)·2025
Same author

Towards a more accurate and reliable evaluation of machine learning protein-protein interaction prediction model performance in the presence of unavoidable dataset biases.

Journal of integrative bioinformatics·2025
Same author

Fcodes update: a kinship encoding framework with F-Tree GUI & LLM inference.

Journal of integrative bioinformatics·2025

Related Experiment Video

Updated: Apr 12, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

1.6K

A comprehensive analysis about the influence of low-level preprocessing techniques on mass spectrometry data for

Hugo López-Fernández, Miguel Reboiro-Jato, Daniel Glez-Peña

    International Journal of Data Mining and Bioinformatics
    |May 8, 2015
    PubMed
    Summary

    Preprocessing Matrix-Assisted Laser Desorption Ionisation Time-of-Flight (MALDI-TOF) mass spectrometry data is crucial. MassSpecWavelet software and Support Vector Machines (SVM) classifiers offer optimal performance for MALDI-TOF data analysis.

    More Related Videos

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
    08:43

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

    Published on: May 11, 2017

    13.1K
    Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
    13:00

    Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

    Published on: April 17, 2012

    14.0K

    Related Experiment Videos

    Last Updated: Apr 12, 2026

    Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
    08:07

    Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

    Published on: September 16, 2025

    1.6K
    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
    08:43

    PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

    Published on: May 11, 2017

    13.1K
    Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
    13:00

    Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

    Published on: April 17, 2012

    14.0K

    Area of Science:

    • Analytical Chemistry
    • Biotechnology
    • Computational Biology

    Background:

    • Matrix-Assisted Laser Desorption Ionisation Time-of-Flight (MALDI-TOF) mass spectrometry generates high-throughput data.
    • Extensive data preprocessing is essential for accurate MALDI-TOF analysis.
    • Key preprocessing steps include baseline correction, smoothing, normalization, peak detection, and alignment.

    Purpose of the Study:

    • To systematically compare different software packages for MALDI-TOF data preprocessing.
    • To evaluate the impact of various preprocessing techniques on classification performance.
    • To identify optimal preprocessing methods and classifiers for MALDI-TOF data.

    Main Methods:

    • Systematic comparison of multiple software packages for MALDI-TOF data preprocessing.
    • Testing various configurations of preprocessing techniques (baseline correction, smoothing, normalization, peak detection, alignment).
    • Training and evaluating classifiers (including Support Vector Machines - SVM) using preprocessed data, assessing performance via kappa and accuracy metrics.

    Main Results:

    • Preprocessing significantly impacts the classification performance of MALDI-TOF data.
    • The MassSpecWavelet software demonstrated superior performance among the tested packages.
    • Support Vector Machines (SVM) emerged as one of the most accurate classifiers for this data.

    Conclusions:

    • Effective preprocessing is critical for successful MALDI-TOF data analysis and classification.
    • MassSpecWavelet is recommended for preprocessing MALDI-TOF data due to its high performance.
    • SVM classifiers provide accurate results when applied to preprocessed MALDI-TOF data.