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Updated: Apr 12, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Mouse embryos' fusion for the tetraploid complementation assay.

Marina Gertsenstein¹

¹Toronto Centre for Phenogenomics (TCP), Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 25 Orde, Toronto, ON, Canada, M5T 3H7, marina.gertsenstein@phenogenomics.ca.

Methods in Molecular Biology (Clifton, N.J.)

|May 8, 2015

Summary

Creating germline-competent mouse chimeras from genetically modified stem cells is crucial for developing new mouse models. This technique aids in studying cell lineage, gene function, and complex phenotypes, particularly using tetraploid complementation assays.

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Area of Science:

Developmental Biology
Genetics
Animal Models

Background:

Genetically modified embryonic stem (ES) cell lines are vital for creating novel mouse models.
Mouse chimeras are essential tools for studying cell lineage and complex mutant phenotypes.
Tetraploid embryos are used to rescue extraembryonic defects and analyze gene function in specific lineages.

Purpose of the Study:

To describe the applications of the tetraploid complementation assay.
To outline the protocol for generating tetraploid embryos and subsequent chimeras.
To highlight the utility of tetraploid complementation in mouse model generation and analysis.

Main Methods:

Generation of tetraploid embryos through blastomere fusion.
Production of germline-competent chimeras using genetically modified ES cells and tetraploid embryos.

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Manipulation of Ploidy in Caenorhabditis elegans

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Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

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Last Updated: Apr 12, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Manipulation of Ploidy in Caenorhabditis elegans

Manipulation of Ploidy in Caenorhabditis elegans

Published on: March 15, 2018

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

Application of the tetraploid complementation assay for various research purposes.

Main Results:

Successful generation of mouse chimeras capable of contributing to the germline.
Demonstration of tetraploid complementation's effectiveness in rescuing extraembryonic defects.
Facilitation of phenotype analysis for mutant mice and gene function studies.

Conclusions:

Tetraploid complementation is a powerful technique for generating mouse models.
This method enables detailed study of gene function and embryonic/extraembryonic tissue interactions.
The described protocol supports the advancement of genetic research and the creation of specialized mouse models.